Subsequently, the nematodes were transferred in buffer consisting of a mixture of methanol and PBT 8. After pretreatment, the nematodes were suspended in phosphate-buffered saline PBS containing 0. Pre-parasitic stage J2s of M. Hybridization signals within the nematode were detected under a light microscope Olympus, Germany. The mycelia of M. The conidia of the transformed M. At least three independent experiments were performed, with four technical replicates for each reaction.
The pepper roots were washed and the number of galls and egg masses were counted 6 weeks after inoculation. At least 30 pepper plants were used for each treatment, and three independent experiments were performed. Transformed T1 seeds were sown on soil and were screened by spraying the herbicide BASTA two weeks later to select for transgenic plants.
The spraying was repeated thrice. Homozygous T3 seeds were collected from T2 lines after a segregation analysis and were used in this research.
Three independent experiments were perfomed. The suppression of cell death elicited by B. The confirmed constructs were then introduced into the B. Nicotiana benthamiana plants were infiltrated with bacterial inocula suspended in a 0. The left half of leaf was injected with B. Cell death was observed within 3 days after infiltration, and leaves were then cleared in ethanol to visualize the HR.
For bacteria population counting, Three 1. Three independent experiments were performed. The preparation of Arabidopsis protoplasts and PEG mediated transfection were performed as previously described All experiment were replicated three times and three independent transformations were used for each replicate. The WT plants were used as negative control. Agrios, G.
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